Table 1.
List of Primers Used
| Tested strains | Locus | Primers | Amplicon size (bp) | Ref |
|---|---|---|---|---|
| EPEC | bfp | F: AATGGTGCTTGCGCTTGCTGC | 326 | 49 |
| R: GCCGCTTTATCCAACCTGGTAAG | ||||
| eaeA | F: GACCCGGCACAAGCATAAGC | 384 | 50 | |
| R: CCACCTGCAGCAACAAGAGG | ||||
| ETEC | lt | F: GGCGACAGATTATACCGTGC | 450 | 49 |
| R: CGGTCTCTATATTCCCTGTT | ||||
| st | F: ATTTTTCTTTCTGTATTATCTTT | 190 | 49 | |
| R: CCGGTACAAGCAGGATTACA | ||||
| EIEC | ipaH | F: ATGCGAGAAATTAATATGCTCAG | 786 | This study |
| R: GAATAGCAGAGTTTGATCTGATAAG | ||||
| virF | F: AGCTCAGGCAATGAAACTTTGAC | 618 | 51 | |
| R: TGGGCTTGATATTCCGATAAGTC | ||||
| STEC | stx1 | F: TTCAGCAAGTGCGCTGGCGA | 212 | This study |
| R: CGCTGAATCCCCCTCCATTA | ||||
| stx2 | F: GGCGCGTTTTGACCATCTTCG | 518 | This study | |
| R: GATGATGGCAATTCAGTATAACG | ||||
| EAEC | aggR | F: GTATACACAAAAGAAGGAAGC | 254 | 51 |
| R: ACAGAATCGTCAGCATCAGC | ||||
| aap | F: GGCATCTTGGGTATCAGCCTG | 313 | This study | |
| R: CCCATTCGGTTAGAGCACTATATT | ||||
| Campylobacter jejuni | hipO | F: ACTTCTTTATTGCTTGCTGC | 323 | pubmlst.org/campylobacter |
| R: GCCACAACAAGTAAAGAAGC | ||||
| Campylobacter coli | F: TCAAGGCGTTTATGCTGCAC | 323 | pubmlst.org/campylobacter | |
| R: CCATCACTTACAAGCTTATAC | ||||
| Campylobacter | 23 rRNA | F: TATACCGGTAAGGAGTGCTGGAG | 650 | pubmlst.org/campylobacter |
| R: ATCAATTAACCTTCGAGCACCG |
EPEC, enteropathogenic E. coli; ETEC, enterotoxigenic E. coli; EIEC, enteroinvasive E. coli; STEC, Shiga toxin-producing E. coli; EAEC, enteroaggregative E. coli; bfp, bundle-forming pilli; eaeA, intimin, effacing and attaching lesion; lt, heat labile toxin; st, heat-stable toxin; ipaH, invasion plasmid antigen; virF, virulence invasion factor; stx1, shiga toxin1; stx2, shiga toxin 2; aggR, aggregative adherence fimbriae I; aap, antigenic antiaggregative protein; hipO, hippurate hydrolysis.