Figure 2. CIB2 in the cochlear hair bundles and the apical surface of hair cells.

• A–C
  Location of CIB2 in F‐actin‐labelled (red) IHCs and OHCs. (A) On P7, specific labelling (absent from CIB2 ^−/− mice, right panels) was observed in the basal body of the kinocilium (open arrowheads) at the apical surface of hair cells, and in the stereocilia, mostly in the basolateral region (arrows). Some unspecific labelling is observed in the supporting cells (asterisk in A). (B, C) On P10 (B) and P20 (C), CIB2 immunostaining was detected in different regions of the stereocilia, including their tips (arrowheads), and at the apical surface of hair cells (arrows), around the cuticular plate.
• D
  Regardless of the stage analysed (P7, P10 or P20), no significant immunostaining was observed in the vestibular hair cells.

Data information: Scale bars: 2 μm.