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. 2017 Oct 16;9(12):1629–1645. doi: 10.15252/emmm.201607445

Figure EV3. Late‐stage glioma M2 macrophages express and present VEGF close to blood vessels.

Figure EV3

  1. vegf in situ hybridization on sections of 5‐week growth glioma in ROSAmTmG ::Pdgfb‐iCre mouse. Tomato‐positive macrophages surrounding blood vessels are positive for the vegf‐specific RNA probe (5‐μm depth stack).
  2. sFlt1 binding assay on section of 5‐week growth glioma in ROSAmTmG ::Pdgfb‐iCre mouse. At late‐stage tumor growth, M2 macrophages surrounding blood vessels present very high amounts of VEGF (binding sFlt1) to the neighboring endothelial cells (50‐μm depth stack).
  3. Quantification of sFlt1 binding accordingly to vessel distance (n = 5 mice).
  4. sFlt1 binding assay together with MHCII and MRC1 immunohistochemistry on section of 3‐week growth glioma in wild‐type mice. sFlt1 binds to MRC1‐positive macrophages, but not MHCII‐positive macrophages (n = 5 mice per group).
Data information: Statistical analysis: (C) one‐way ANOVA followed by multiple comparisons Tukey's test. Error bars: mean ± SD. Scale bars: (A) 50 μm; (B) 250 μm; (D) 150 μm.