Figure EV5. NLRP11 is induced and translocates to mitochondria by type I IFNs.

• A–C
  NLRP11 mRNA expression in THP‐1 monocytes and HeLa cells infected with Sendai virus (SeV) (MOI = 1), or transfected with poly(I:C) (10 μg/ml) or treated with IFN‐β (1,000 U/ml) for indicated time points was analyzed by real‐time PCR. Data are expressed as means ± SEM of three independent experiments (*P < 0.05, **P < 0.01, and ***P < 0.001, versus untreated cells, Student's t‐test).
• D
  293T cells were transfected with GFP‐NLRP11, and then, these cells were left untreated (UT) or were transfected with poly(I:C) (5 μg/ml) for 12 h, followed by confocal fluorescence microscopy analysis. Mitochondria were detected with mitochondria tracker (red). Scale bar, 20 μm.