Fig. 6.

E2F1 promoted cell migration and invasion in ccRCC cells. a RNA expression of E2F1 and normal tissues was detected by RT-PCR in ccRCC tissues (n = 30, P < 0.01). The expression of E2F1 was normalized to GADPH. b The protein expression of E2F1 after knockdown was detected in ccRCC cells by western blot. c, d Cells migration and invasion ability and cells number were detected after E2F1 knockdown in ccRCC cells (P < 0.05). All data were represented as the mean ± SD from three independent experiments, **P < 0.01