Fig. 2.

Effects of miR-214 mimic transfection in Jurkat cells apoptosis. a Remarkable elevation of miR-214 expression levels in Jurkat cells after transfection with miR-214 mimic versus controls (transfected with scramble oligonucleotides); (b) increased Jurkat cells apoptosis after cultured with TNF-α (20 ng/mL) for 7 days, compared with culture medium alone; (c) in Jurkat cells transfected scrambled oligonucleotides, the apoptotic rate of Jurkat cells was increased after cultured with TNF-α (20 ng/mL) for 24 h compared with those cultured with medium alone. The apoptotic rate was similar in Jurkat cells transfected with miR-214 mimic cultured either in the presence or absence of TNF-α (Fig. 2c)