Table 1.

Sample experimental data from a single qPCR plate for analysis. Hypothetical data are used to show the results of a plate experiment examining the expression of a gene of interest (g) and two reference genes (ref1 and ref2) for two sample types, A and B. The controls for the plate experiment are not shown. $Mean{C}_q^{(w)}$ represents the the arithmetic $Mean{C}_q^{(w)}$ across the three technical replicates

	Well efficiency (E)	Gene	C q	log(E)	log(E) ∙ C q	$Mean{C}_q^{(w)}$
Sample type A	1.844	g	31.246	0.266	8.303	8.397
Sample type A	1.843	g	31.490	0.265	8.360
Sample type A	1.836	g	32.316	0.264	8.527
Sample type B	1.839	g	32.565	0.265	8.647	8.611
Sample type B	1.834	g	32.782	0.263	8.631
Sample type B	1.823	g	32.802	0.261	8.555
Sample type A	1.905	ref1	26.645	0.280	7.458	7.336
Sample type A	1.886	ref1	26.618	0.276	7.335
Sample type A	1.868	ref1	26.579	0.271	7.215
Sample type B	1.918	ref1	26.101	0.283	7.382	7.353
Sample type B	1.906	ref1	26.096	0.280	7.309
Sample type B	1.915	ref1	26.105	0.282	7.368
Sample type A	1.900	ref2	26.191	0.279	7.298	7.180
Sample type A	1.881	ref2	25.983	0.274	7.129
Sample type A	1.879	ref2	25.962	0.274	7.113
Sample type B	1.890	ref2	25.308	0.277	6.998	7.054
Sample type B	1.883	ref2	25.256	0.275	6.940
Sample type B	1.911	ref2	25.689	0.281	7.223
					Sample type A Efficiency-weighted ΔC q	${\mathrm{\Delta }C}_{q;r,A}^{\left(w\right)}=$1.1387
					Sample type B Efficiency-weighted ΔC q	${\mathrm{\Delta }C}_{q;r,B}^{\left(w\right)}=$1.4077