Table 2.

Results of unpaired t-test. An unpaired t-test and 95% confidence interval are calculated in SPSS assuming unequal variances using the hypothetical data from Table 1 and three other hypothetical plate experiments. The P-value is from a two-tailed test assuming a mean difference of 0

	Sample type A ${\mathrm{\Delta }C}_{q;r,A}^{\left(w\right)}$	Sample type B ${\mathrm{\Delta }C}_{q;r,B}^{\left(w\right)}$
r = 1	1.1387	1.4077
r = 2	0.845	1.291
r = 3	0.499	1.496
r = 4	0.699	1.172
Mean ${\mathrm{\Delta }C}_q^{\left(w\right)}$	0.7954	1.3417
SD	0.269	0.141
N	4	4
${\mathrm{\Delta \Delta }C}_q^{\left(w\right)}$			−0.546
T for ${\mathrm{\Delta \Delta }C}_q^{\left(w\right)}$			−3.60
P			0.019
95% CI for ${\mathrm{\Delta \Delta }C}_q^{\left(w\right)}$			(−0.949, −0.143)
Estimated Expression Ratio ${10}^{-\mathrm{∆∆}{C}_q^{\left(w\right)}}$			3.52
95% CI for ${10}^{-\mathrm{∆∆}{C}_q^{\left(w\right)}}$			(1.33, 9.29)