Table 6.

Results of improper t-test usage. An improperly implemented paired t-test using hypothetical data from Table 1 and three other hypothetical plate experiments testing the hypothesis of equal gene expression between sample type A and B assuming a mean difference of 0

Biological replicate r	Sample A ${\mathrm{\Delta }C}_{q;r,A}^{\left(w\right)}$	Sample B ${\mathrm{\Delta }C}_{q;r,B}^{\left(w\right)}$	${{10}^{-\mathrm{\Delta \Delta }C}}_{q;r}^{\left(w\right)}$ A vs. B	${{10}^{\mathrm{\Delta \Delta }C}}_{q;r}^{\left(w\right)}$ B vs. A
r = 1	1.1387	1.4077	1.858	0.538
r = 2	0.845	1.291	2.793	0.358
r = 3	0.499	1.496	9.931	0.101
r = 4	0.699	1.172	2.972	0.337
		Mean	4.39	0.333
		SD	3.73	0.180
		N	4	4
		T	1.82	−7.42
		P	0.167	0.005