Table 9.

Primers used in this study

Primers	5′ to 3′ sequencea	Use
XIS1_460109ApaUpF	NNNNNNGGGCCCCAGGATATGCCATTCAGC	Mutant construction
XIS1_460109BamUpR	NNNNNNGGATCCCAATGACATCAGGCACAC	Mutant construction
XIS1_460109BamDnF	NNNNNNGGATCCGAACCATCGCAGATTGAG	Mutant construction
XIS1_460109XbaDnR	NNNNNNTCTAGAGCCCAATCGCTTCATATC	Mutant construction
XIS1_460115ApaUpF	NNNNNNGGGCCCGAATCGCCCTGGATTATG	Mutant construction
XIS1_460115BamUpR	NNNNNNGGATCCCCCTCTGGCTGATAATAG	Mutant construction
XIS1_460115BamDnF	NNNNNNGGATCCCTCAGGCTCGATTATTGG	Mutant construction
XIS1_460115XbaDnR	NNNNNNTCTAGACTGAATGTACTCCTGCTG	Mutant construction
NilBF	NNNCATATGAGGAAAACGCCACATTCCGG	Confirmation PCR
NilBR	NNNGGGCCCTTGCATGGTTTGGTTG	Confirmation PCR
M13F (−20)	GTAAAACGACGGCCAG	Sequencing PCR
M13R	CAGGAAACAGCTATGAC	Sequencing PCR

^aN represents A, T, G or C. Engineered restriction enzyme sites are underlined