Table 3.
Post-inoculation rectal swab PEDV N-gene RT-qPCR and swine bioassay results
| Treatment Group | Day 3 Rectal Swab; Individual CT results (genomic copies / mL) | Day 3 Rectal Swab; Percentage positive for PEDV RNA | Day 7 Rectal Swab; Individual CT Results (genomic copies / mL) | Day 7 Rectal Swab; Percentage positive for PEDV RNA | Swine Bioassay Result; Percentage positive for PEDV RNA |
|---|---|---|---|---|---|
| (A) Negative Control | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (B) Positive Control | 16.2 (109.14)
15.1 (109.46) 16.8 (108.96) 15.0 (109.49) |
100% (4 of 4) | 22.2 (107.37) 15.2 (109.43) 19.3 (108.23) 17.4 (108.79) |
100% (4 of 4) | 100% (4 of 4)b |
| (C) Light, 1:32, 40 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (D) Heavy, 1:32, 40 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (E) Light, 1:16, 40 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (F) Heavy, 1:16, 40 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (G) Light, 1:32, 60 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (H) Heavy, 1:32, 60 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (I) Light, 1:16, 60 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
| (J) Heavy, 1:16, 60 mins | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | >35 (0) >35 (0) >35 (0) >35 (0) |
0% (0 of 4) | 0% (0 of 4)a |
a,b Different superscripts indicate statistically significant differences (p-value <0.05) by a Fishers Exact Test