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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Sci Signal. 2016 Aug 23;9(442):ra83. doi: 10.1126/scisignal.aaf5642

Fig. 6. DNMT1 or DNMT3A knockdown increases excitability, decreases the mAHP, and decreases expression of SK channel–encoding genes.

Fig. 6

Neurons were exposed to scrambled control, Dnmt1 ASO, or Dnmt3a ASO. (A) Relative Dnmt1 and Dnmt3a mRNA expression after ASO exposure (scrambled control, n = 9 biological replicates; Dnmt1 ASO, n = 9 biological replicates; Dnmt3a ASO, n = 9 biological replicates). (B and C) Representative immunoblot (B) and relative DNMT1 and DNMT3A protein abundance (C) after ASO exposure (scrambled control, n = 9 biological replicates; Dnmt1 ASO, n = 9 biological replicates; Dnmt3a ASO, n = 8 biological replicates). (D and E) Representative recordings of evoked firing responses (D) and mean firing rates (E) of ASO-treated neurons (scrambled control, n = 12 cells; Dnmt1 ASO, n = 9 cells; Dnmt3a ASO, n = 7 cells). (F) Representative recordings of single APs from ASO-treated pyramidal neurons overlaid to show the mAHP. (G) Graph of mAHP amplitudes after ASO exposure (scrambled control, n = 9 cells; Dnmt1 ASO, n = 5 cells; Dnmt3a ASO, n = 5 cells). (H) Relative transcript expression of the SK channel genes Kcnn1, Kcnn2, and Kcnn3 after ASO exposure (scrambled control, n = 9 biological replicates; Dnmt1 ASO, n = 9 biological replicates; Dnmt3a ASO, n = 9 biological replicates). (A, C, and H) Data are normalized to scrambled control and are means ± SEM from three experiments. (E and G) Graphs show means ± SEM from cells pooled from at least three experiments for each condition. (A, C, G, and H) *P < 0.05, one-way ANOVA followed by Dunnett’s test. (E) *P < 0.05, RM-ANOVA.