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. 2017 Dec 1;13:370. doi: 10.1186/s12917-017-1295-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1. — Prevalence and copy numbers of C. suis affected by samples type of pigs. Copy number (± SEM) is shown in the left ordinate (in red). Positivity is shown in the right ordinate (in blue). FRET-qPCR were performed to determine the positivity and copy number of C. suis from nasal, conjunctival, rectal swabs and whole blood samples of 50 sows and 50 weaned pigs in this study. The positivity of C. suis in nasal swabs (66%), conjunctival swabs (63%) and rectal swabs (60%) was significantly higher than whole blood (P<0.01, 8%). The average copy number of C. suis was highest in rectal swab (P<0.01, 10^{5.34±0.16 [SEM]} per swab), followed by conjunctival swab (10^4.39±0.14 per swab) and nasal swab (10^4.37±0.14 per swab), and whole blood (P<0.05, 10^3.50±0.20 per ml).