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. 2017 Dec 1;13:370. doi: 10.1186/s12917-017-1295-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2. — Prevalence and copy numbers of C. suis affected by ages of pigs. Copy number (± SEM) is shown in the left ordinate (in red). Positive rate is shown in the right ordinate (in blue). FRET-qPCR were performed to determine the positivity and copy number of C. suis from nasal swabs of suckling pigs (n=324), weaned pigs (n=312), finisher pigs (n=100) and sows (n=195) in this study. The positivity of C. suis was the lowest in sow (P<0.01, 31.3%), followed by finisher pigs (84.0%) and weaned pigs (84.0%), and suckling pigs (P<0.05, 69.4%). The average copy number of C. suis was significantly higher in weaned pigs (P<0.01, 10^{3.88±0.06 [SEM]} per swab) than sows (10^3.11±0.09 per swab), finisher pigs (10^3.09±0.07 per swab) and suckling pigs (10^2.94±0.05 per swab).