Table 2.
ompA and MLST primers used in this study
| Chlamydia | Target | Primer/probe | Sequence (5'-3') | Amplicon Size (bp) | MLST fragment (bp) |
|---|---|---|---|---|---|
| C. suis |
Cs_ompA
VD 1-2 |
UP | TTGAACATTTGGGATCGTTTTGA | 491 | _ |
| DN | CCAATGTAAGGAGTGAACATATTTAATCTG | ||||
| gatA | UP | TAAAAGTGCTTTAGAATTAAGAGATGCTGT | 539 | 425 | |
| DN | AGATGCTGGCTGACGAATCGA | ||||
| oppA_3 | UP | AGATATCAGTGGGAATCTGCTTGC | 674 | 468 | |
| DN | TAAGGATTTTTTTCCAATTTAAGCCAT | ||||
| hflX | UP | CTCTCCCTCTCAACAACGGAACTT | 616 | 435 | |
| DN | TTCAATAACATGCAGCAAAATATCCTC | ||||
| gidA | UP | TTTGGGAGTTTCTACGAAGGAAGG | 570 | 474 | |
| DN | ATAATTTCATATTGTACATCGAAAGGCAT | ||||
| enoA | UP | TCTCGGGGTCTCTTTAGCATTAGC | 590 | 381 | |
| DN | CTCCAACGAGTTGAATACGATCTCC | ||||
| hemN | UP | GAAGAGCTTGCTATTGAATTTGATCC | 608 | 432 | |
| DN | CGTTTTGTAGATAGATTCCTCGAATGA | ||||
| fumC | UP | CTCTTATGGGAAAGAATTGATGCCT | 641 | 465 | |
| DN | TACTTTCTCTACAAAACCTTCAGGAACATT | ||||
| C. abortus | Cab_ompA | UP | TACAAGCCTTGCCTGTAGGGAAC | 360 | _ |
| DN | CAGAAAATATCAAAGCGATCCCAG | ||||
| C. pecorum | Cpe_ompA | UP | ATGAAAAAACTCTTAAAATCGGCGT | 420 | _ |
| DN | CAGAAAATATCAAAGCGATCCCAG |