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IVC system used in the current study. (A) Cage air-flow diagram. HEPA-filtered air is supplied through sealed shelf plenums, directly through an air supply orifice located above the cage filter top. Air is exhausted through an exhaust-air orifice, also located above the filter top. (B) Mouse cage with filter top. Approximate exhaust site (circle) used for sample collection from the filter top. Reproduced with permission from the manufacturer (Thoren Caging Systems).
