Figure 2.
Effect of SRB on the quantity and distribution of fecal output from healthy guinea pigs. (A) The cumulative (n = 4) fecal output diagram for each day (1 d [green], 2 d [blue] and 3 d [red] )in both females and males in all groups. In the day-1 diagram for the females in the sham group, dashed boxes were added to indicate the 4 equal quadrants inside the cages. The cumulative total areas from both sexes and all time points were used to compare the total fecal output areas between treatment groups and quadrants. (B) With the exception of females in the sham group (all time points) and SRBLow group (day 2) and males in the SRBLow group (day 3), the total fecal output areas were lower than those for males in the sham group, independent of the dose of SRB tested (‡, P < 0.001, 2-way ANOVA). The red dotted line represents the fecal output quantity during the acclimation period (baseline). (C) Changes in the fecal output distribution after administration in the sham and SRB groups were significant only in quadrant 4 (Q4). Different uppercase letters represent significant differences between Sham and SRB groups (P < 0.001, Tukey test). Cage dimensions, 40 × 20 cm; Scale bar, 12.5 cm.