Table 2. The primers used to measure gene expression levels for the candidate reference genes by RT-qPCR.
| Gene | GenBank accession number | Primer sequence (5′to 3′)a | Amplicon length (bp) | Tm (°C)b | E (%)c | R2d |
|---|---|---|---|---|---|---|
| GAPDH | KY786279 | F: GCCATTCCAGTAAGTTTTCCATTGAG | 157 | 85.0 | 100.75 | 0.91 |
| R: GCTGTTACTGCTACACAAAAGAC | ||||||
| TUB1a | KY786273 | F: CAGACTGCACGTTGGACTTTAGC | 172 | 83.6 | 100.04 | 0.96 |
| R: TACAGAGGAGATGTTGTCCCCAAG | ||||||
| EF1a | KY786281 | F: AAACCTTTGCGTCTTCCACTACAGG | 184 | 81.7 | 99.83 | 0.94 |
| R: CTTCAGTTGTAAGACCAACAGGTG | ||||||
| EF2 | KY786280 | F: GATGAGAAATCCACATGTCCAG | 244 | 82.0 | 102.00 | 0.86 |
| R: CGACTCCCTAGTATCAAAGG | ||||||
| ARF1 | KY786283 | F: GTATGACAGTAGCTGAAGTTC | 141 | 81.4 | 112.70 | 0.84 |
| R: CTGTTTTGTAAAGCATTGGC | ||||||
| ARF4 | KY786282 | F: TAGTACGGACGGTCAAGTC | 197 | 89.1 | 105.91 | 0.81 |
| R: GTAGACCGTCACCTGTTATGGC | ||||||
| RPS8 | KY786274 | F: CATTATGTACGTACGAGAGGAGGCAACG | 200 | 84.0 | 99.96 | 0.91 |
| R: TCTAAAGGGAGTAGCGTCGATAACG | ||||||
| RP4 | KY786275 | F: TAATGGACCACGACGCTGTATGC | 248 | 84.5 | 100.33 | 0.92 |
| R: CGTACCAGCTTTAGTAATGAGCAAGG | ||||||
| L7A | KY786277 | F: TAGCGACTCAACTGTTCAAGG | 224 | 84.8 | 99.54 | 0.95 |
| R: CCTCAATTGGATCGACGTCATGTG | ||||||
| L10 | KY786278 | F: CGTAGAGCCTCGATAACTTGG | 210 | 84.7 | 99.33 | 0.94 |
| R: TCATGTGCTGGAGCTGATAGG | ||||||
| RP18 | KY786276 | F: TTGTAACCACATGAACGCCTACG | 186 | 85.2 | 99.75 | 0.96 |
| R: AGTTAGCTTTACGTTCACCTACTGG |
Notes.
a
F, forward primer; R, reverse primer.
b
melting temperature.
c
real-time qPCR efficiency (calculated by the standard curve method).
d
regression coefficient (calculated from the regression line of the standard curve).