Fig. 1.

Effects of X-rays on cell viability of RAW264.7 cells. (A) Typical morphology of RAW264.7 cells 4 h and 2 days after exposure to various doses of X-rays. Scale bar, 100 μm. (B–C) The CCK 8 method was used to evaluate the cell viability relative to control in the presence or absence of RANKL (n = 3). (D) IR increased caspase 3 activity in both RAW264.7 and differentiating osteoclasts on Day 2 and 4 (n = 3). (E) IR did not altered caspase 3 activity in RAW264.7 cells at 4 h or on Day 1 (n = 3). All X-ray groups were compared with controls. Data shown are in the form of mean ± SD. *P < 0.05.