Table 1.
Primers sequences used for quantitative real-time PCR
| Gene name (Genebank No.) | Primer sequences (5′–3′) | Annealing temperature (°C) |
|---|---|---|
| Car2 (NM_009801.4) | Forward: CATTACTGTCAGCAGCGAGCA | 54 |
| Reverse: GACGCCAGTTGTCCACCATC | ||
| CTSK (NM_007802.4) | Forward: CAGCAGAACGGAGGCATTGA | 54 |
| Reverse: CCTTTGCCGTGGCGTTATAC | ||
| Integrin β3 (NM_016780.2) | Forward: CCACCTTCACCAATATCAC | 55 |
| Reverse: CCAAATCCCACCCATACAC | ||
| MMP9 (NM_013599.3) | Forward: GCCCTGGAACTCACACGACA | 56 |
| Reverse: TTGGAAACTCACACGCCAGAA | ||
| GAPDH (NM_008084.2) | Forward: TGCACCACCAACTGCTTAG | 51 |
| Reverse: GGATGCAGGGATGATGTTC | ||
| RANK (NM_009399.3) | Forward: CTTGGACACCTGGAATGAAG | 52 |
| Reverse: CAGCACTCGCAGTCTGAGTT | ||
| V-ATPase (NM_175406.3) | Forward: CCACTGGAAGCCCAGTAAACAGA | 55 |
| Reverse: GAACGTATGAGGCCAGTGAGCA |