Figure 6.
Knockdown of HN1L could not induce cell apoptosis and senescence. (a) Apoptosis cells were analyzed by flow cytometry (Annexin V-FITC/PI staining). Data represent mean ± SD. (b) Western blot analyzed the levels of several apoptosis associated proteins in sh-HN1L, sh-control and parental cells. β-Actin was used as a loading control. (c) Cell senescence was tested by β-Galactosidase (β-Gal) staining. Representative β-Gal positve cells were indicaed by red arrows. Scale bar, 50 μm. Data represent mean ± SD. (d) Western blotting showed the protein level of p16Ink4a in A549 and HCC827 cells before or after HN1L silencing. β-Tubulin was used as a loading control.