Figure 1.
Mw suppresses proliferation and invasion of B16F10 cells. (A) MTT assay of Mw (dose; 0, 104 −108 cells/ml) for 24hr and 48hr on melanocyte, B16F10 and B16F1 cells were analyzed. The experiment was repeated thrice and expressed as mean ± SD.π P< 0.05,ππ P< 0.01; *P< 0.05, **P< 0.01 versus untreated for 24hr and 48hr. (B) Effects of Mw on cell viability were assayed by Trypan blue exclusion assay for 24hr and 48hr. The experiment was repeated thrice and expressed as mean ± SD.ππ P < 0.01; **P < 0.01 vs. untreated for 24hr and 48hr. (C) Antiproliferative effect of Mw for 24hr and 48hr were measured by [3 H]–Thymidine incorporation. Triplicate results were expressed as mean ± SD.ππ P < 0.01; **P < 0.01 versus untreated cells for 24hr and 48hr. (D) Clonogenicity of B16F10 and B16F1 cells treated with Mw was assessed by soft agar colony assay. Results were expressed as mean ± SD. *P < 0.05, **P < 0.001 vs untreated. (E) Invasion assay was carried out in 12-well transwell after Mw treatment for 2hr. The randomly chosen fields were photographed (20X), and the number of cells migrated to the lower surface was calculated. Data are mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.001 vs untreated. (F) Confluent cells were treated with Mw and scratched. After 24hr, the number of cells migrated into the scratched area was photographed (20X) and calculated. Data are mean ± SD of 3 independent experiments. *P< 0.05, and **P< 0.001 vs untreated. (G) Cell adhesion was carried out in a 12-well plate coated with matrigel and treated with Mw for 2hr. Attached cells were photographed (20X) and calculated. Data are mean ± SD of 3 independent experiments.*P< 0.05, **P< 0.001 vs untreated.