Four examples of parent GUVs containing inner vesicles, in the presence of
daptomycin (2 μM, added outside). No daptomycin is
observed on the inner vesicle membranes. In each example, the left panel shows
the fluorescence of the lipid probe LRh-DOPE (red), which was incorporated in
the membrane during the GUV preparation. The right panels show the daptomycin
fluorescence, from its intrinsic kynurenine chromophore (cyan). Daptomycin is
present in the outer membrane of the parent GUV, but not in the inner
vesicles.