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Rhodamine (LRh-DOPE) and daptomycin fluorescence distributions coincide on membranes of POPC:POPG (80:20), containing 0.1 mol% LRh-DOPE and 2 mM Ca2+. The daptomycin concentration in the outside solution was 2 μM. (A) All fluorescence channels (green, carboxyfluorescein; red, LRh-DOPE; cyan, daptomycin). (B) Rhodamine fluorescence only (red). (C) Daptomycin fluorescence channel only (cyan).
