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. 2017 Oct 3;8(53):90638–90650. doi: 10.18632/oncotarget.21469

Figure 3. HSF1 is overexpressed and strongly activated in c-Myc mice.

Figure 3

A. HSF1 mRNA levels as assessed by reverse-transcription real-time PCR. Number target (NT) = 2-ΔCt, wherein ΔCt value of each sample was calculated by subtracting the average Ct value of the HSF1 gene from the average Ct value of the β-actin gene. B. HSF1 activity, as determined using the HSF1 ELISA kit (Enzo Life Sciences), following the manufacturers’ instructions. Tukey–Kramer test: P < 0.0001 a, vs control (mice injected with empty vector); b, vs c-Myc mice. Ten mice per each group were analyzed.