Figure 4.
A. Islet cells from IKO and floxed mice under HFD/NC treatment were grown for 6 h and then stained with Mitotracker green and Mitotracker deep red or MitoSOX for 30 min and analyzed by flow cytometry. B.The IL-1b mRNA expression levels treated by siNLRP3 and Bay11-7082. C. Relative mRNA expression levels of the proinflammatory cytokines TNF-α, IL-1β, IL-6, IL-10 and MCP-1 were determined by qRT-PCR in islets from IKO and floxed mice. Mean±SD from 3 independent experiments, analyzed by one-way ANOVA following Newman-Keuls post hoc test. **,P < 0.01 compared to the control groups.