Figure 2. CPS-mediated inhibition of enzymatic proteasome activity is ROS-, Ca²⁺- and TRPV1- dependent.

A. Enzymatic proteasome activities were assessed in WT thymocytes pre-treated with NAC, EDTA and CPZ for 60 minutes and then treated with CPS for 120 minutes. Data, shown as percentage of proteasome activity, are from one representative experiment out of three separate experiments. Error bars are relative to three replicates. *p < 0.01 vs untreated cells; **p < 0.01 vs CPS-treated cells. B. Western blot analysis and densitometric quantification of p27 protein levels in thymocytes treated as above described. p27 densitometric values were normalized to GAPDH used as loading control. Blots are representative of one of three separate experiments. #p < 0.01 vs untreated; **p < 0.01 vs CPS-treated cells.