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. 2017 Oct 11;8(53):90766–90780. doi: 10.18632/oncotarget.21798

Figure 5. Knock-out of TRPV1 gene affects autophagy, proteasome and UPR protein expression in thymocytes.

Figure 5

A. Western blot analysis and densitometric quantification of basal p62 protein levels in WT and TRPV1 KO thymocytes. Densitometric values were normalized to GAPDH used as loading control. Blots are representative of one of three separate experiments, *p < 0.01 vs WT thymocytes. B. Western blot analysis and densitometric quantification of LC3 and p62 protein levels in TRPV1 KO thymocytes treated for up to 4 h with rapamycin. Densitometric values were normalized to GAPDH used as loading control. Blots are representative of one of three separate experiments, *p < 0.01 vs CPS-treated cells for 0.5h or untreated cells. C. Thymocytes from WT and TRPV1 KO mice were subjected to enzymatic proteasome activity assay. Data shown as percentage of inhibition are representative of one of three separate experiments, *p < 0.01 TRPV1 KO vs WT thymocytes. D-I. Western blot analysis and densitometric quantification of p27 D., pAMPK/AMPK E., ATF-4 F., ERp57 G., Bip H. and Grp94 I. protein levels in WT and TRPV1 KO thymocytes. Densitometric values were normalized to GAPDH used as loading control. Blots are representative of one of three separate experiments, *p < 0.01 vs WT thymocytes.