Table 1. CPS induces UPS gene expression changes.
Ref Seq | Symbol | Description | Fold change CPS-treated vs untreated |
---|---|---|---|
NM_007527 | Bax | Bcl2-associated X protein | -2.04±0.03* |
NM_013760 | Dnajb9 | Dnaj (Hsp40) homolog, subfamily B, member 9 | -2.09±0.08* |
NM_022310 | Hspa5 | Heat shock protein 5 | -2.79±0.02* |
NM_153526 | Insig1 | Insulin induced gene 1 | -3.31±0.02* |
NM_172307 | Mbtps2 | Membrane-bound transcription factor peptidase, site 2 | -2.10±0.02* |
NM_133933 | Rpn1 | Ribophorin 1 | -2.40±0.04* |
NM_013842 | Xbp1 | X-box binding protein 1 | -2.67±0.05* |
NM_013558 | Hspa1l | Heat shock protein 1-like | -2.27±0.43* |
NM_024207 | Derl1 | Der1-like domain family, member 1 | +2.65±0.03* |
NM_033562 | Derl2 | Der1-like domain family, member 2 | +2.09±0.02* |
NM_026184 | Ero1lb | ERO1-like beta (S.Cerevisiae) | +2.10±0.04* |
RT-PCR array in mRNA samples from thymocytes untreated and treated with CPS for 60 min. The expression levels were normalized using the housekeeping gene GAPDH and calculated by the ∆∆Ct method. Data, shown as fold change, represent the mean ± SD of three experiments. Untreated thymocyte mRNA was used as calibrator. *p < 0.01 vs untreated cells.