Figure 1. OX40L-FP enhances CD4⁺ T-cell proliferation and inhibits Treg suppression.

A., In-vitro CD4⁺ and CD8⁺ T-cell proliferation assays. CD4⁺ and CD8⁺ T-cells isolated from WT Balb/c mice were co-cultured with irradiated APCs and various concentrations of soluble anti-CD3 and OX40L-FP. H³-thymidine uptake was measured after 4 days in culture. B., Treg suppression assay. CD4⁺ responder cells from Balb/c mice were co-cultured with varying amounts of syngeneic Tregs, and stimulated with soluble anti-CD3, 10 µg/mL OX40L-FP, and irradiated APCs. H³-thymidine uptake was measured 3 days later.