Figure 4. Combination therapy augments the total CD4⁺Foxp3- population, and CD4 Tem and Tcm subsets, while enhancing Twist-specific T-cell responses in 4T1 tumor-bearing mice.

A., Absolute number of CD4⁺Foxp3^- T-cells in the lungs, spleens, and DLN (n = 5) on day 28 post tumor transplant. B., Representative contour plots (denoting frequencies) and absolute numbers of CD4⁺ Tem (CD44⁺CD62L^-) and Tcm (CD44⁺CD62L⁺) populations. Events were pre-gated on CD3⁺CD4⁺Foxp3^- T-cells. C., Twist-specific CD4⁺ T-cell proliferation assay. CD4⁺ T-cells pooled from lungs and spleens on day 28 post tumor transplant were stimulated with MHC-II–restricted Twist peptide in the presence of irradiated APCs. H³-thymidine uptake was measured 4 days later.