Figure 5. Combination therapy increases CD4⁺ T-cell infiltration into 4T1 metastatic sites of the lung.

A., Representative immunofluorescence images of CD4 (green) and nuclear (blue) staining at 10x magnification. B., H&E and Twist stains demonstrating co-localization of Twist expression with regions of 4T1 metastasis in the lung. C., Representative images of CD4 staining (bottom panels) in areas of the lung positive for Twist (top panels).