Skip to main content
. 2017 Aug 24;8(53):90842–90851. doi: 10.18632/oncotarget.20412

Figure 2. Cyclin A2 depletion induces DNA double-strand breaks and defects in HR repair.

Figure 2

A. After 48 hr siRNA transfection, MCF-7 and MDA-MB-231 cells were immunostained for γ-H2Ax and 53BP1, and cells with >5 γ-H2Ax/53BP1 co-localized foci were counted to measure the incidence of DSBs. B. Control (luciferase) siRNA- or cyclin A2 siRNA-transfected MCF-7 cells were exposed to ionizing irradiation (1 Gy) and the efficiency of DNA DSB repair was examined by counting cells with >5 γ-H2Ax/53BP1 co-localized foci after 24 hr. C. The cells were transfected with control luciferase (Luc), or cyclin A2 siRNAs. 48 hr after transfection, the cells were irradiated with 10 Gy of ionizing radiation and stained to detect RAD51 foci. D. MCF-7-DR-GFP cells transfected with pCβASceI plasmid plus control luciferase (Luc), or cyclin A2 siRNAs were analyzed by flow cytometry for GFP fluorescence after 72 h transfection. Data in (D) were normalized to luciferase-transfected cells for each experiment. Mean ± SEM., n = 3; **P < 0.001, Unpaired t test.