Figure 3. Andrographolide synergistically enhanced the anti-proliferative effects of As₂O₃ on HepG2 cells via downregulation of EphB4.

(A) Heat map showing the effect of andrographolide plus As₂O₃ on the expression of HepG2 genes involved in andrographolide’s synergistic anti-cancer activity. (B) Quantitative RT-PCR was used to evaluate the levels of EphB4, Ras and VEGFR1 in HepG2 cells. (C) The expression of EphB4 and VEGFR1 were analyzed by western blot. Each experiment was repeated independently more than three times.