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. 2017 Jul 20;8(53):91052–91066. doi: 10.18632/oncotarget.19409

Figure 1. Asparaginase induced cytotoxicity and apoptosis in GBM cells in vitro.

Figure 1

(A) Cells were collected and lysed, then western blot analysis was performed to assess the expression level of ASNS in U87MG and U251MG cells. 95D cells were used as a positive control, while Jurkat cells were used as a negative one. (B, C) U87MG and U251MG cells were incubated with different concentrations of asparaginase for 12, 24 and 48 h, then cell viability was measured by MTT assay. (D) U87MG cells were treated with 1, 2, 4 IU/mL of asparaginase for 48 h, and stained with Annexin V/PI, then analyzed by flow cytometry. (E) U251MG cells were treated with 2, 4, 8 IU/mL of asparaginase for 48 h, and stained with Annexin V/PI, then analyzed by flow cytometry.