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. 2017 Jul 20;8(53):91052–91066. doi: 10.18632/oncotarget.19409

Figure 2. Asparaginase-induced apoptosis was related to caspase 3 activation in GBM cells.

Figure 2

(A, B) U87MG and U251MG cells were dose- and time-dependently treated with asparaginase, then western blot analysis was performed to assess the expression level of PARP, cleaved PARP and cleaved-caspase 3. (C, D) U87MG and U251MG cells were incubated with 0.5 IU/mL of asparaginase, either alone or in combination with 20 μM z-VAD-fmk for 24 h, then western blot analysis was performed to assess the level of PARP, cleaved PARP and cleaved-caspase 3. (E, F) U87MG and U251MG cells were treated with asparaginase at indicated concentrations in the absence or presence of 20 μM z-VAD-fmk for 24 h, then cell viability was determined by MTT assay at the wavelength of 570 nm. Results were represented as mean ± SD (*P < 0.05).