Figure 5. The Akt/mTOR and Erk signaling pathway participated asparaginase-induced autophagy in GBM cells.

(A, B) U87MG and U251MG cells were dose- and time-dependently treated with asparaginase, the level of mTOR, p-mTOR, p-p70S6K, Akt, p-Akt and p-4EBP1 were analyzed by western blot analysis. (C, D) U87MG and U251MG cells were dose- and time-dependently treated with asparaginase, the level of Erk1/2 and p-Erk1/2 were analyzed by western blot analysis.