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. 2017 Jul 20;8(53):91052–91066. doi: 10.18632/oncotarget.19409

Figure 6. ROS was involved in autophagy by asparaginase in GBM cells.

Figure 6

(A–D) U87MG and U251MG cells were treated with 0.5 IU/mL of asparaginase, either alone or in combination with 5 nM NAC for 48 h. (A, B) Cells were stained with MitoSOX and Cyto-ID® Green autophagy dye and examined by confocal fluorescent microscopy. (C, D) Autophagy-associated protein LC3-I/II were detected by western blot analysis. (E, F) U87MG and U251MG cells were treated with asparaginase at indicated concentrations either alone or in combination with 5 nM NAC for 48 h, then cell viability was determined by MTT assay at the wavelength of 570 nm. Results were represented as mean ± SD (*P < 0.05, **P < 0.01).