Figure 2. Modulation of [Ca²⁺]c and [Ca²⁺]ER by three regulators of Ca²⁺ signaling in rPT cells.

Cells were treated with 0.5 µM Pb for 12 h in the presence or absence of 10 µM 2-APB (co-incubation for the entire course), 1 µM TG (co-incubation for the entire course) and 10 µM BAPTA-AM (30 min pre-incubation before Pb treatment) to assess the changes of [Ca²⁺]_c and [Ca²⁺]_ER, assessed by flow cytometry. Values of fluorescence intensity of Fluo-3 (A) and Mag-Fluo-4 (B) are quantified in a relative way to its respective control, whose value is set at one. Data represent mean ± SEM (n = 6). ns not significant, ^*P < 0.05, ^**P < 0.01.