Figure 5. Effect of BAPTA-AM on Pb-induced protein levels of ER stress markers in rPT cells.

Cells were pretreated with 10 µM BAPTA-AM for 30 min, then exposed to 0.5 µM Pb for another 12 h to measure the protein levels of GRP78 (A), GRP94 (B), CRT (C), CHOP (D) and caspase-12 (E). Upper panel representative western blot image; lower panel quantitative analysis (mean ± SEM, n = 4). ^*P < 0.05, ^**P < 0.01.