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. 2017 Aug 24;8(53):91209–91222. doi: 10.18632/oncotarget.20436

Figure 4. Evaluation of autophagy by monitoring ATG-5, -7, and the conversion LC3B-I to LC3B-II in whole protein extracts from HeLa, SiHa, and CaSKi cells treated with WGA at the indicated concentrations.

Figure 4

(A) ATG-5 and -7 expression increased in all cervical carcinoma cells following treatment with WGA at the indicated concentrations. (B) Western blot showing dose-dependent expression of LC3B in HeLa, SiHa, and CaSKi cells treated with WGA for 24 h. The cytoplasmic form of LC3 (LC3B-I) and the autophagosomal membrane-bound form (LC3B-II) were both detected. We quantified the relative levels of LC3-II to β-Actin. Bars represent the mean ± SD of three independent experiments (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (C) MTT assays in HeLa, SiHa, and CaSKi cells treated with the indicated concentrations of WGA in the presence and absence of Baf-A1, an inhibitor of autophagy. Bars represent the mean ± SD of four independent experiments (P > 0.05, not significant; ***, P < 0.001).