Figure 4. Impact of melatonin on PI3K/Akt/mTOR, HIF-1α synthesis pathway assayed by Western blot.

(A) Cells were incubated under hypoxia (Hx) for 0, 1, 3 and 6 h with or without melatonin (2 mM). Lane 1 of each panel shows normoxic basal protein levels. (B) and (C) Effect of rapamycin (20 nM) and LY294002 (50 μM) alone or in combination with melatonin on HIF-1α and PI3K/Akt/mTOR-related proteins measured by Western blot.