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. 2017 Oct 10;8(53):91674–91683. doi: 10.18632/oncotarget.21748

Figure 4. Hsa_circ_000984 may serve as miR-106b sponge to regulate its circRNA-miRNA-mRNA network.

Figure 4

(A) The expression levels of CDK6 in CRC tissue samples and their paired normal samples. The result showed that CDK6 expression in CRC tissue was significantly higher than those in corresponding nontumorous tissues (*P<0.05, paired t-test). (B) A significantly positive correlation was found of hsa_circ_000984 expression level with CDK6 expression level. CRC cell lines were transfected with hsa_circ_000984-shRNA or hsa_circ_000984-pCDNA-3.1 vectors, respectively. *P<0.05, two-side Student’s t-test. The expression of CDK6 were monitored by qRT-PCR (C) and western blotting assays (D). (E) Schematic illustration of biological roles of hsa_circ_000984 in CRC carcinogenesis. Hsa_circ_000984 can bind to miR-106b as a miRNA sponge, exerting its function via regulating the downstream target CDK6, and knockdown of hsa_circ_000984 could inhibit CRC cell proliferation, migration, invasion in vitro and tumor formation in vivo, respectively.