Table 2.

HPLC assays conditions.

Columna	Drug	Mobile phase	Flow rate (ml/min)	Injection volume (μl)	Detection (nm)	Retention time (min)	R2b	LOQ (μM)
2	Indomethacin	Mobile phase A: Ammonium formate 10 mM pH 3.0 in H2O Mobile phase B: Ammonium formate 10 mM pH 3.0 in ACN/H2O (9:1 v/v)	1	10	254	0.84	0.9919	0.31
2	Phenytoin		1	10	260	2.3	0.9961	51
1	Felodipine		1	10–50	260	3.1	0.9997	9.4
1	Fenofibrate		1	10	291	3.7	0.994	0.94
1	Probucol		1	10–50	254	4.5	0.9999	2.5
1	Aprepitant		1	10	254	3.0	0.9995	26
2	Tadalfil		1	10	291	2.1	0.998	1.9
2	Zafirlukast		1	10	260	2.9	0.9991	0.27
2	Carvedilol		1	10	254	1.0	0.9999	9.0

Apparatus Agilent Technologies 1260 Series Liquid Chromatography system with Clarity Chromatography software: Gradient method: Time 0, 70%A:30%B, 3 min 0%A:100%B, 4 min 0%A:100%B, 4.5 min 70%A:30%B total run time 8 min. ACN: acetonitrile. LOQ: Limit of Quantification.

^aColumn 1 Hichrom ACE 3 C18/DV148262/50 × 3.0 mm id/ACE-111-0503/A149937: Column 2 Hichrom ACE 3 C18/SIN-A46224/50 × 2.1 mm/ACE-111-0502/A46224.

^bR² Linear regression coefficient of calibration curve, n = 5 or 6 points.