Fig 7.

(A) Analyses of Pitx2c expression in Pitx2 gain and loss-of-function experiments with or without H₂0₂ administration for 12h and 24h, respectively. Observe that H₂0₂ administration significantly decreased Pitx2c expression in both Pitx2 overexpression and silencing conditions, at 12h and 24h. (B) Analyses of Cat and Sod2 expression (B), Prdx2 and Prdx5 (C) and Wnt8a, Wnt11 and Zfhx3 (D) in Pitx2 gain and loss-of-function setting with H₂0₂ administration for 12h and 24h, respectively. Observe that Cat is significantly increased by Pitx2 overexpression at 12h and 24h whereas is mildly increased and highly significantly decreased by Pitx2 siRNA treatment at 24h. On the other hand Sod2 is mildly decreased only at 24h in both conditions (B). Prdx5, but not Prdx2, is significantly decreased at 12h and 24h in both conditions (C). Wnt8a is only increased in Pitx2c siRNA conditions at 12h, Wnt11 is increased in both conditions only at 12h while Zfhx3 is significantly increased only by Pitx2c silencing at 24h. Representative values of three pooled replicates on three independent biological transfections. *p<0.01, **p<0.05, ***p<0.001, ****p<0.0001.