Fig 8.

Analyses of Scn5a, Kcnj2 and Cacna1c (A), miR-1, miR-29a, miR-106b and miR-200b (B) expression in Pitx2 gain and loss-of-function experiments after H₂0₂ administration for 12h and 24h, respectively. Observe that most ion channel are significantly decreased in both experimental conditions at both time points (A) whereas microRNAs are all significantly decreased after Pitx2 over-expression and increased following Pitx2c silencing at 12h and 24h after H₂0₂ administration. (C). miR-29a and miR-200 expression in HL-1 atrial cardiomyocytes transfected cells. Observe that each microRNAs is significantly increased after corresponding pre-miRNA transfection. (D) Ion channel gene expression in miR-29a and miR-200 transfected HL-1 atrial cardiomyocytes, respectively. Observe that miR-29 and miR-200 over-expression leads to significant decreased of Cacna1c, Hcn4, Ryr2 and Camk2a (except for miR-29a) expression. Representative values of three pooled replicates on three independent biological transfections. *p<0.01, **p<0.05, ***p<0.001, ****p<0.0001.