Figure 2.

Quantitative real time PCR (qRT-PCR) analysis of the expression of selected miRNAs and their target mRNAs during Edwardsiella tarda infection (A) and validation of miRNA targets (B). (A)Tongue sole were infected with E. tarda for 0 h, 12 h, and 24 h, and miRNA and mRNA expressions in spleen were determined by qRT-PCR. Expression levels relative to 0 hpi were expressed as bars. Bars stacked together indicate no negative correlation between miRNAs and mRNAs. The names of the mRNAs and miRNAs are shown above and below the bars, respectively. (B) 293T cells were co-transfected with different miRNA mimics and their target mRNA-3′-UTR reporter plasmids. Relative luciferase activity was measured at 24 h after transfection. The experiments were performed 3 times, and values are shown as means ± SEM.