Effect of Eaf2 loss on heterozygous p53 mice. (A) Prostates of mice on a p53+/− background developed high-grade mPIN lesions at 17 to 20 months of age. (black arrow, Eaf2+/−p53+/− inset) and increased vessels (red arrow, Eaf2+/−p53+/− inset). Hematoxylin and eosin (H&E) staining of mouse ventral prostate. Original magnification, ×10, inset, ×40. Scale bars, 200 μm in ×10, 50 μm in ×40. (B) Ki-67 immunostaining in transverse sections of prostate anterior lobes from indicated genotypes at 17 to 20 months of age. Original magnification, ×20. (C) Quantification of Ki-67+ epithelial cells in prostate at 17 to 20 months of age. Data are expressed as mean ± standard error of the mean from 7 to 12 mice per group. *P < 0.05, ***P < 0.001.