Figure 8.

The ΔramA mutant exhibits altered susceptibility profiles to antifungal drugs. The E-test method was used to delineate minimum inhibitory concentrations (MICs) for the wild type (WT) and ramA deletion mutant (ΔramA) on GMM and RPMI agar. Antifungals tested were Amphotericin B (AP), Caspofungin (CS), Itraconazole (IT) and Voriconazole (VO). A small but consistent increase in susceptibility was noted for the ΔramA mutant in the presence of AP. In contrast, moderate and consistent increases in MIC values were noted for the ΔramA mutant in the presence of the triazoles, IT and VO. No changes in susceptibility were noted for the cell wall targeting compound, CS.