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. 2017 Apr 19;8(7):1303–1315. doi: 10.1080/21505594.2017.1321191

Figure 1.

Figure 1.

Ifnb induction by NTM mycobacteria in macrophages. (A) qPCR for Ifnb in RAW cells infected with viable MAP 6783, MAP K10, MAH 1287, MAA 44156, MAA 44158, M. bovis BCG Pasteur or M. smegmatis mc2 (MSM) (MOI 5) for 5 h. (B) IFN-β ELISA of supernatant from RAW cells infected with MAP or MSM. (C) qPCR for Ifnb in RAW cells and (D) BMDM infected with viable (V) or heat inactivated (HI) MAP or MSM (MOI 5) for 30 min (’), 2 h and 5 h. (E) RAW cells and (F) BMDM were infected with MAP or MSM (MOIs 2.5, 5.0, 7.5, 10) for 5 h. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Tukey post test, means ± SEM. (G) RAW cells and (H) BMDM were pre-treated with 5 µM latrunculin B (Lat) for 1 h and subsequently infected with MAP or MSM for 5 h. Data are representative of 3 independent experiments, *p<0.05, **p<0.01 by 2 tailed student's t-test means ± SEM.